Allogene Therapeutics (NASDAQ:ALLO) Q1 2026 Earnings Call

0:00 Hello, thank you for standing by and welcome to Allogene Therapeutics First Quarter twenty twenty six Conference Call. After the speakers' presentation, there will be a question and answer session. To ask a question during the session, you will need to press 11 on your telephone. You will then hear an automated message advising your hand is raised. To withdraw your question, please press 11 again. Please be aware that today's conference call is being recorded. I would now like to turn the call

0:30 over to Christine Cassiano, Chief Corporate Affairs and Brand Strategy Officer. Ms. Cassiano, please go ahead. Thank you, operator, and welcome, everyone, to Allogene's conference call. After the market closed, Allogene issued a press release that provided a business update and financial results for the first quarter of twenty twenty six. This press release and today's webcast are available on our website. Following our prepared remarks, we will host a Q and A session and will aim to keep the call to under an hour. I am joined today by Doctor. David Chang, President and Chief Executive Officer Doctor. Zachary Roberts, Executive Vice President of Research and Development and Chief Medical Officer and Jeff Parker, Chief Financial Officer.

1:17 During today's call, we will be making certain forward looking statements. These may include statements regarding the success and timing of our ongoing and planned clinical trials, data presentations, regulatory filings, future research and development efforts, manufacturing capabilities, the safety and efficacy of our product candidates, commercial market forecasts, potential treatment settings, and financial guidance, among other things. These forward looking statements are based on current information, assumptions, and expectations that are subject to change. A description of potential risks can be found in our press release and latest SEC disclosure documents. You are cautioned not to place undue reliance on these forward looking statements, and Allogene disclaims any obligation to update these statements. I'll now turn the call over to David.

2:05 Thank you, Christine. As we move through 2026, next generation cell therapy is shifting from promise to proof. The field is increasingly being defined by differentiated clinical evidence rather than platform ambition alone. At Allogene, our lead program SEMICELL is built around the clear objective to establish a differentiated development path.

2:30 That strategy is now translating into data that provides support for our approach. Our second program, ALLO-three thousand two and ninety in autoimmune indications is built on the same principle of product differentiation enabled by our understanding of CAR T design and the biology of allogeneic rejection. While these two programs are at different stages of development, the evidence emerging to date is consistent and aligned with the design principles behind each. Starting with Alpha-three, we have taken an innovative approach of treating patients with Semisel in the first line consolidation setting for large B cell lymphoma, with a primary goal of improving the cure rates. The key to achieving this goal is democratizing access by breaking the barriers that have historically limited the use of CAR T therapy and by enabling SemiCell to be delivered in the outpatient setting.

3:28 We are very pleased with what we've seen in the recently announced interim futility analysis from the ALPHA-three trial. In this 24 patient analysis, SemiCell achieved a 58.3 MRD clearance rate compared with sixteen point seven percent in the observation arm, representing a forty one point six percent absolute difference. While preliminary, this differential exceeded threshold of MRD clearance reported in other trials that led to groundbreaking clinical outcomes. We also observed a rapid and substantial reduction in circulating tumor DNA or ctDNA in the stem cell arm, while the opposite trend was seen in the observation arm where the ctDNA levels increased.

4:16 Together, these early findings provide evidence consistent with the biological activity of stem cell in the first line consolidation setting as we advance ALPHA3 towards the next key milestone, the interim EFS analysis in mid-twenty twenty seven. Importantly, as we consider use in the outpatient community setting, this early biomarker efficacy signal was accompanied by a favorable safety profile. We observed no CRS, ICANS or treatment related hospitalization, enabling the majority of patients to be managed in the outpatient setting. These results reflect the trial that was designed to lead, not follow. Under Zach's leadership, ALPHA-three was built around MRD testing as a point of intervention rather than passive observation, an approach that moved beyond conventional trial design.

5:13 We set out to test that forward looking thesis and these early data reaffirm my conviction that we are not only in the right path, but ahead of the curve. Taken together, we believe these data provide compelling support for a different paradigm. One where SEMISA can be used earlier, made readily available, delivered broadly, and potentially integrated into routine care beyond specialized centers. Turning to ALLO-three 29, the program is progressing through early clinical development in autoimmune indications with the resolution basket trial advancing efficiently through dose escalation. This progress embodies the same discipline and forward looking development approach that underpins ALPHA-three.

6:03 ALLO-three 29 incorporates the DAGGER technology, which is designed to overcome premature rejection of allogeneic CAR T cells. This technology has previously been validated as part of our ALLO-three sixteen program in the metastatic solid tumor setting. However, autoimmune disease represents a fundamentally different clinical context with distinct biology and the different threshold for safety and tolerability. With that in mind, we designed a structured and stepwise clinical approach beginning at a conservative dose level to establish clear understanding of tolerability before progressing to therapeutic dose levels. Patients treated today are within this initial dosing range as we evaluate both dose and lymphodepletion strategy.

6:52 Our focus is on characterizing how the therapy behaves in patients by establishing a tolerability profile that supports continued development, while also assessing early signs of activity.

7:05 Within this framework, we are very pleased with the pace of enrollment and are beginning to observe initial signs of clinical activity coupled with favorable tolerability. Whilst too early, these findings are highly encouraging and have important implications for the overall dosing paradigm, which includes not only the dose of dagger enabled ALLO-three twenty nine, but also the required lymphodepletion regimen. As the program progresses, we expect continued dose escalation and patient follow-up to further establish the activity, tolerability and mechanistic profile of ALLO-three twenty nine. We look forward to providing a further update in the fourth quarter.

7:50 With that, I will turn it over to Zach to walk through the data in more detail. Thanks, David. I'll start with ALPHA3 and then turn to ALLO-three 29. ALPHA3 was designed around a clear clinical hypothesis that intervening at the point of molecularly detectable disease before clinical relapse can meaningfully alter the course of disease. When we initiated the study, MRD was emerging as a prognostic tool in LVCL. Our objective was to move MRD beyond risk assessment and into a treatment decision point. Across oncology, we are now seeing the shift in approaching a potential breakout moment. A case in point is the INVIGURE 11 trial, which evaluated Tecentriq in muscle invasive bladder cancer. In the trial, patients who were in remission but remained MRD positive after the standard first line procedure of complete surgical resection were randomized to Tecentriq or placebo with Tecentriq demonstrating improvement in both disease free and overall survival.

8:51 The results of this trial could establish MRD as a clinically actionable endpoint following standard first line treatment. If approved for this indication to centric would become the first therapy for which treatment initiation is guided by an ultra sensitive ctDNA MRD assay rather than clinical progression, a defining moment for the field. Against this backdrop, alpha-three is positioned at the forefront of how this new paradigm could evolve in large B cell lymphoma as the first pivotal trial designed to use MRD positivity as the trigger for CAR T therapy. The ALPHA-three study is enrolling patients who have responded to first line therapy, but remain MRD positive and therefore at high risk of relapse. Patients are randomized to treatment with Senocell or observation.

9:37 We partnered with Foresight, now a wholly owned subsidiary of Natera, to utilize their CLARITY MRD assay, enabling a highly sensitive and dynamic view of disease burden over time. This enhanced sensitivity detecting disease at or even below one in a million or 10 to the minus six is central to the design of ALPHA-three study and how we interpreted our interim futility data. At the interim analysis, we evaluated the first 24 patients enrolled in the ongoing two arms, a single dose of Semi cell versus observation. We observed a fifty eight point three percent MRD clearance rate in the Semi cell arm compared to a sixteen point seven percent in the observation arm, representing a 41.6 percentage point absolute difference.

10:23 We also saw a rapid and substantial reduction in circulating tumor DNA. At the day 45 time point, the median ctDNA level decreased by nearly 98% in the semisil arm, while the median ctDNA level increased by more than 26% in the observation arm. The ALPHA-three interim futility analysis rests on the assumption that MRD clearance foreshadows clinical benefit. This hypothesis is supported by a growing body of evidence in various clinical settings, including in LBCL, linking MRD clearance in the range of twenty five percent to thirty percent with meaningful reductions in EFS events. The magnitude of the difference we just announced exceeds that range. While these external data sets support the relationship between MRD clearance and clinical outcomes, the impact on EFS and durability will ultimately be determined through our planned interim and primary EFS analyses.

11:18 From a safety and treatment administration perspective, we observed no CRS, ICANS or treatment related hospitalizations, enabling the majority of patients to be managed entirely in the outpatient setting. We believe this encouraging tolerability profile is a function of treating patients earlier when disease burden is low, which is inherent to the ALPHA-three design. If the safety profile observed in the interim futility analysis bears out in the study overall, it could mark an important shift towards outpatient CAR T administration and enable semiselle treatment in community practices, where most patients with LBCL receive care. As alpha-three progresses, interest in the study is growing. First and foremost, we are seeing robust engagement from existing clinical sites resulting in high rates of patient screening.

12:06 At the same time, new sites are expressing significant interest in joining the study further reinforcing its momentum. From an execution standpoint, the trial continues to scale. We are now enrolling across more than 60 sites with global expansion underway. We recently announced regulatory approval in Australia and South Korea, where site activations and patient screening have begun. We anticipate the Asia Pacific region to expand the study footprint to over 80 sites worldwide. These are not incremental additions. Australia and South Korea offer established clinical research infrastructure, experienced investigators, and highly efficient healthcare systems. This expansion reflects both strong global investigator interest and the operational discipline required to execute at scale.

12:53 We are also seeing meaningful participation from community cancer centers, which contributed approximately one third of screening and semiselle treatments in our interim futility analysis. This is an important early proof point for the feasibility of broader administration as we look to move beyond specialized centers and into broader clinical practice. Let me now turn to ALLO-three 29, which as a first in human Phase one trial has a different objective at this stage of development. The program is supported by robust preclinical data recently published in Nature Communications, supporting the design of ALLO-three 29. These data demonstrated an optimized CD70 CAR engineered to protect allogeneic CAR T cells from rejection by eliminating alloreactive host T cells.

13:40 In those studies, co expression of CD70 and CD19 CARs drove sustained CAR T cell persistence elimination of pathogenic B cells and activated CD70 positive T cells in humanized SLE models and corresponding reductions in autoantibody production. Importantly, the dagger technology which eliminates alloreactive host T cells has been clinically validated by our third clinical program and first CD 70 targeting program, allo three sixteen with recently reported outcome data further supporting the approach and reinforcing our plans to advance the program in the near future. At this stage of development, our focus for ALLO-three twenty nine is to define a tolerability profile that supports continued dose escalation while generating early evidence that ALLO-three twenty nine can achieve meaningful biological activity in autoimmune disease consistent with its differentiated dual targeting mechanism.

14:37 The resolution basket trial which includes patients with systemic lupus erythematosus with and without nephritis, scleroderma and inflammatory myositis continues to progress through dose escalation. Nine patients have already been treated since the study started enrollment in November 2025. With three patients at dose level one of twenty million cells, and three patients at dose level two of forty million cells, both following lymphodepletion with cyclophosphamide, and three patients at dose level one with no lymphodepletion. Importantly, the doses evaluated so far are substantially lower than those being explored in other CAR T approaches in autoimmune disease, including autologous programs testing approximately one 100,000,000 cells, and some allogeneic approaches evaluating doses above 1,000,000,000 cells.

15:26 Even at these lower doses of ALLO-three twenty nine, both with and without cyclophosphamide, investigators have reported signs of clinical activity. While these observations are preliminary and dose exploration continues, investigators have been very encouraged by these early signals, facilitating strong patient interest in participating in the study. This reflects a consistent approach across our programs. In ALPHA-three, we designed the study to intervene earlier in disease treatment based on MRD. With ALLO-three 29, we are applying that same forward looking discipline to autoimmune disease prioritizing mechanism, durability, scalability, and long term usability from the outset. As we continue dose escalation and patient follow-up, our goal is to build a data set that integrates clinical activity with mechanistic understanding and supports a path towards durable outcomes.

16:17 We expect to provide a comprehensive update in the fourth quarter. Across both programs, our focus remains consistent, designing studies with clear hypotheses, executing with discipline, and allowing the data to define the role of allogeneic CAR T. With that, I'll turn it over to Jeff. Thank you, Zach. As we execute against our key clinical milestones in 2026, we remain focused on maintaining a strong financial position that supports continued progress across our portfolio. As of March 31, we had 266,900,000 in cash, cash equivalents and investments. In April, we strengthened that position through a public offering that generated approximately $200,400,000 in gross proceeds, extending our cash runway into the first quarter of twenty twenty nine.

17:11 R and D expenses for the first quarter were $32,000,000 including $2,700,000 of non cash stock based compensation, reflecting continued investment in our clinical programs.

17:24 G and A expenses for the first quarter were $14,100,000 including $5,600,000 in non cash stock based compensation. Net loss for the first quarter was $42,600,000 or $0.18 per share, including non cash stock based compensation expense of $8,300,000 Based upon our current forecast for the overall timing of the Alpha-three program, we are modestly increasing our guidance for operating cash expense in 2026 from approximately $150,000,000 to $165,000,000 GAAP operating expenses are also expected to slightly increase from approximately $210,000,000 to $225,000,000 including estimated non cash stock based compensation expense of approximately $35,000,000 These estimates exclude any impact from potential business development activities.

18:25 Overall, we believe we are well positioned to execute on our strategy with the capital and flexibility needed to reach our next set of milestones. We'll now open the call for questions. Thank you.

18:58 And our first question comes from Michael Yee of UBS. Your line is open. Hey, guys. Congrats on the progress to date and the updated autoimmune color. Maybe two quick ones. One is, can you talk a little bit more specifically about some of the initial signs of activity or B cell reductions? What does that mean? And to what degree maybe there are differences in B cell reductions for the lymphodepletion cohort compared to any of the different cohorts with different lymphodepletion? And if I may get a question on obviously the lead DLBCL program. Since the announcement of your MRD negativity interim, how have you seen perhaps enrollment, engagement and feedback and things of that nature? Maybe just talk a little bit about how things have progressed since that positive interim. Thank you.

19:58 Hey, Mike. This is Zach here. Thanks for the questions. So on the three twenty nine question, we are we'll be saving the details to sort of pertinent to your question until the Q4 update other than to say that the encouraging signs that we refer to are coming from the cohorts that we've highlighted both with and without LD. So we will be continuing to enroll patients according to the protocol design with and without cyclophosphamide and expect to show

20:31 a more complete update in Q4. But so far, so good and we're really thrilled with the patients coming into the study very briskly. The second question around has the IA1A results stimulated increased activity in ALPHA-three, I will say that they have. It in these early days, these first couple of weeks since the announcement went out, that has come in the form of new sites coming and asking to participate in Alpha-three, even sites that said early on that they didn't have room in their portfolio before the IA1A data was available. Now they're coming back and saying that they really do want to participate. So that kind of qualitative change is already underway.

21:19 We will be watching very carefully for a quantitative uptick in the screening and enrollment pace, except I will say that that has been going very well in the last few months. So we're optimistic, but so far we're pretty happy with the way things are going. Great. Thank you. Thank you.

21:43 And our next question comes from Tyler Van Buren of TD Cowen. Your line is open. Hey guys, congrats on the progress. I have a couple three to nine questions as well. Since you mentioned favorable tolerability, can you discuss conceptually what sort of safety profile you hope to achieve with ALLO-three twenty 9 over the long term? And then with respect to the update in the fourth quarter, can you give us any sense of what that might entail and kind of help put goalposts around what we should expect with that update?

22:20 Hey, Tyler, thanks for those questions. The safety profile in autoimmune indications, I mean, we want this to be as clean as one can get to. I mean, I think that's really the patient population that we're dealing with. I we have seen in ALPHA-three study even in oncology in the right clinical setting, CAR T therapy can be well tolerated. And that's kind of profile that we are trying to mirror where the treatment can be given as an outpatient and patient can be managed as an outpatient. So that's really the safety profile that we're looking for. And so far after completing both twenty million and forty million cell dose levels with cyclophosphamide lymphodepletion, I feel very encouraged that

23:15 if the safety profile holds out, this can be very interesting finding. With respect to your second question about how to set up the expectations for the fourth quarter. I mean, so far, let's keep in mind in terms of the pace of enrollment that Zach had talked about. We dosed first patient back in November of last year and May. So within six months, dose escalation study where we have to wait about a month after first patient is dosed before we can fill up the rest of the court with even with that kind of sort of

23:59 preset barriers in how fast we can enroll, we enrolled nine patients. That is a pretty remarkable support that we are getting from various physicians involved in the autoimmune trials. So we are highly encouraged.

24:20 Autoimmune, obviously, the way that the clinical responses are being measured is different than in oncology. But when the investigators are calling us and telling us that they're seeing that they would not have expected to see in any setting, I mean that I would view as a highly encouraging early signs.

24:43 Thank you. And our next question comes from Biren Amin of Piper Sandler. Your line is open. Yes, hi guys. Thanks for taking my question. Maybe just to stay on ALLO-three 29. Given your reporting data in Q4, should we expect data across the 20,000,040 cell doses in the fourth quarter? Or could we get higher cell doses? So that's the first question. Second question, what would be the patient composition across SLE, myositis and sclerosis in the Q4 update?

25:21 And then lastly, on the trial itself, recently in April, I think there was a change that was recorded on ct.gov where you increased target enrollment to 66 patients from 54 patients. Could you maybe just talk about that change and what drove that? Thank you.

25:40 Biren. As to your first question, I'm also realizing that was Tyler's last question, which I did not fully answer. So in the fourth quarter, obviously at this point, we have completed twenty million and forty million and we are continuing the dose escalation by the fourth quarter update comes included patient in that will be more than just twenty million and forty million. We're hoping that we can include certainly the next dose level and possibly even in a higher dose depending on how the pace of enrollment is maintained. And also when I'm talking about the cell dose levels, certainly I'm talking about both with site and without cyclophosphamide.

26:27 And Zach, maybe you can cover the patient composition. Yes. So, Biren, we're seeing patients come from all of the indications that I listed previously. So lupus, inflammatory myopathies and scleroderma. It's still too early to say whether the early signs of efficacy that we are seeing is segregated to one patient group or another. So we're not going to be making any changes to the basket style design of protocol. So as we continue to enroll patients, we expect a mix. And that mix will be presented in Q4. And then the ct.gov change that occurred,

27:12 I actually have to say I don't know why we made that It's an administrative change. Yeah, administrative change. Sometimes we have to go through and we have to make little clerical changes to the ct.gov. There's not been any changes to the study design that would have warranted that change.

27:32 Thank you. And our next question comes from Salveen Richter of Goldman Sachs. Your line is open. Good afternoon. Thanks for taking my question. For stem cell, could you speak to the feedback you're hearing from the community practices to date post the recent data? And then for the ongoing ALLO-three 29 resolution study, can you expand upon progress on-site activation and patient enrollment and how that's playing out just given some competition in the field for other autoimmune CAR T programs? Thank you. Delveen, it's Zach. So as far as how the community practices are reacting to the interim data from SemiCell, I would echo what I said a moment ago that the feedback has been really overwhelmingly and uniformly positive.

28:27 Just to give another little anecdote on that, in the couple of literal days after that announcement was made that same week, we were on the phone with a couple of large community network practices that have already a couple of sites on the study seeking to expand their footprint within their practices and add additional sites. So we believe that the interim analysis data is being viewed by the community practices

28:58 and academic practices alike as potentially something very, very interesting and practice And also I would say it makes a lot of sense. And when you think about this is giving the community physicians something to offer to patients rather than referring them to a tertiary or cell therapy centers. And then two, I think during the course of treating patients, they are realizing that treatment is relatively hassle free.

29:27 And what we have shared with the interim fertility findings is the early safety profile. It was very clean, which also makes managing patient after the CAR T infusion extremely smooth. I mean, are definitely early findings, but these are the things that I believe is making the community physicians being quite interested in participating in the ALPHA-three study.

29:56 And then your second question, Salveen, about site activation and enrollment in three twenty nine. We are approaching the target number of sites that we sought to activate for ALLO-three 29. I can't say exactly what the number is on ct.gov, but we expect that to be completed here very shortly within the next few weeks, maybe a couple of months. That's gone very well. We've actually ended up

30:23 getting quite a bit more traction even in that competitive space that you highlighted. We've got some really excellent marquee sites already listed on ct.gov and a few more in the can ready to come out. And then they are really being super productive on the patient screening and enrollment as we highlighted previously. So we could not be happier with how ALLO-three twenty nine is going operationally. And the mixture of the patients in this basket study is essentially is excellent. Thank you. And our next question comes from Samantha Semencal of Citi. Your line is open. Good afternoon. Thanks very much for taking the question. Another one on 329.

31:14 I'm wondering if you could just talk a little bit about your thoughts on dosing going forward.

31:19 You mentioned some early clinical signals that you're seeing in these first nine patients. I'm wondering, do you think the twenty million doses is too low or sub therapeutic? Curious your thoughts there. And how are you thinking about dosing going forward? Are there any adjustments you're planning to make to the dose escalation?

31:38 Thanks very much. Hey, Sam. So with respect to the question around the doses that we've tried so far, we in the tenets of Phase one dose escalation study, you're really gated by safety. And I want to echo what David said a moment ago that safety is paramount here. We want to make sure that we maintain the safety profile that would make this therapy attractive to patients and doctors with autoimmune disease. And so we're going to keep going up until we start to bump up against that, the toxicity that we think would be prohibitive. So whether you call it sub therapeutic or room to go, I'm more of a room to go type of guy.

32:24 And so we're going to keep dose escalating. And then your other question is, do we see any need to make any adjustments? At this point, we do not. We still have some doses to go up. And we're hopeful that we'll start to see really compelling activity here in these next dose or the dose after that and we can maintain that safety profile. So look forward to that Q4 update. Thank you.

32:55 And our next question comes from Roger Song of Jefferies. Your line is open. Hi, this is Chacha on for Roger. Thanks so much for taking our question. I have two. So one is, can you just give us some more color on what your current cash runway covers? And then on my second question is on also ALLO-three 29.

33:16 Can you tell us more about the decision factors that are driving the optionality for the fludarabine addition? I know you mentioned with the option of adding this, can you just explain the gating factors for why we add or why we don't? Thanks. Hi, this is Jeff. On the cash runway, as we discussed in the script, our current cash runway based on the addition of the capital added in the recent financing, the $200,000,000 financing, takes us into the first quarter of twenty twenty nine. And during that time, we intend to complete the ALPHA-three study. So as you know, we anticipate finishing enrollment at the end of twenty twenty seven.

34:01 We anticipate an interim analysis on EFS in mid-twenty seven, primary analysis in mid-twenty eight with the filing of the BLA as quickly as possible based on the results of those interim or final analysis. So it really is focused on covering ALPHA-three as well as completing this phase one resolution study for 03/29, as we indicated, having the comprehensive data set in the fourth quarter of this year.

34:34 And let me take the question on the optionality of fludarabine. I think this is just how we try to make the protocol as flexible as possible without predefined idea about when to kick in this optionality. I mean, it's really looking at the data and then making the decision. And right now, based on the data, our primary focus is continuing the dose escalation.

35:04 And I'll just add one other piece of color on that. And honestly, there in part, would be driven by the demand to come into the study. By opening additional cohorts, it would allow us to park some patients and to allow patients into the trial. So that is another factor that could come into it. But nothing has changed at all about our belief in the Si or no Si possibility here. So we are continuing with that primary goal. Thank you. And our next question comes from Matthew Phipps of William Blair. Your line is open. Good afternoon. Thanks for taking my question. You know a lot it's going be on three gs-nine as well.

35:50 There's obviously been a lot made about B cell reset after CAR T with C-nineteen. I was wondering if from your experience with ALLO-three sixteen, what the T cell kind of repopulation might have looked like after treatment if you

36:06 looked at that? And I guess, do you expect maybe some ability to have an immune auto reactive T cell reset following three twenty nine treatment? Will you have any data on that by Q4? Thank you.

36:20 Matt, a great question, really insightful and I think right on point in terms of our understanding of the mechanism of action of VALO-three twenty nine. We are really focused on how the B cell and the T cell repertoires will change. And whether we achieve the reset as defined as just absolute B cells going all the way down to zero or whether there's some editing of the repertoire on both the B cell and the T cell side. Now obviously, B cells, we expect the chances of those cells going very low or to zero to be higher because we're targeting a pan B cell marker. In the case of CD70, you're absolutely correct. It's only a subset that are going to be CD70 positive.

37:08 Of course, there's going to be a population of alloreactive cells that we expect to be depleted through the dagger effect. But there is also known to be CD70 positive pathogenic AID causing T cells, so clonal populations with specific TCRs that also express CD70. And that has actually been one of the main reasons that we designed three twenty nine the way we did, was try to wipe out that that clone or set of clones that may be driving the pathogenesis. And so we will be analyzing the T cell repertoire as part of the ALLO-three 29 study. And if we've got something to share in Q4, we'll share it. Thank you. And our next question comes from Jack Allen of Baird. Your line is open.

37:58 Great. Thanks so much for taking the questions and congrats on the progress. I'll keep the theme going with three twenty nine questions and start, and then I have one on stem cell as well. On three twenty nine, was just hoping you could provide some additional color as it relates to how many doses you could escalate the study in and what the next step might be. I guess logically, it looks like sixty million would be a realistic target for the next dose, but how high could you go in that trial? And then on SEMICEL, I just wanted to ask about the interim EFS look in mid-twenty seven and any additional color you can provide around the powering there? So Jack, great question. The next dose level that we're going to be looking at is not 60, it's actually 80,000,000 cells. So that cohort is enrolling now following Cy and then the no Cy cohort is close behind.

38:52 I'm going to hold off on saying exactly how many cell doses that we plan to go up And we'll sort of reveal that at Q4 because I do think that we'll be in that zone by the time we give that update. So stay tuned. But eighty million is the next dose cohort that we're working on now. As far as Semi Cell, we talked quite a bit about this with the fundraising and the alpha allocation for the IA2. We have not gone into specific detail, the method that we use to allocate the alpha with the O'Brien Fleming spending function. So that will give you some general understanding of the fraction of alpha that's allocated to the IA two. I will take the opportunity now to reiterate though that the results of the interim analysis one does give us the possibility of having a positive outcome in IIA II. And so that is something we are looking forward to and of course working feverishly to enroll the study and deliver on those timelines.

39:58 Awesome. Thanks so much for the color. Thank you. And our next question comes from John Newman of Canaccord Genuity. Your line is open. Hi, guys. Thank you for taking the question. Also have one on ALLO-three 29. The question is, do you expect that the dagger technology that's targeting CD70 positive T cells could actually give you maybe differential efficacy in some of the cohorts that you're enrolling versus lupus, for example, scleroderma and myositis where it's sort of theorized that there's more T cell activity? Thanks. John, great question. As usual, thank you for the insight.

40:45 And the answer is yes. So not just within these rheumatologic disorders where T cells are known to play a role and there's literature on it. But of course there are lots and lots of papers and understanding about other therapeutic areas, which are even thought to be more dependent on T cell biology, just to throw out a couple of multiple sclerosis and type one diabetes, are thought to be primarily driven by pathogenic T cells. And so not only within this initial set do we think we could have some differential efficacy, as you put it, but we also believe it will give us a more plausible pathway into other therapeutic areas where T cells are known to play a larger role than just a straight CD19 product.

41:31 Great. Thank you. Thank you. And our next question comes from Reni Benjamin of Citizens. Your line is open. Hey, guys. Thanks for taking the questions. Maybe just starting off with the interim analysis. Now that you've been able to, you know, kind of sit on the data with futility analysis, do you feel that this delta that you're seeing increases the chance of a successful interim mid-twenty twenty seven versus what you were thinking when you first started the study? And if so, does it make sense to potentially modify the trial design to allocate a little bit more alpha and increase your chances of success there?

42:16 And then regarding 03/29, as we kind of look at the landscape, look at autologous therapies and bispecifics, can you maybe just guide us as to what is the clinical sort of efficacy and safety benchmarks you're hoping to hit, hoping to meet, so that you can move this program forward? And does this program move forward ideally with a partner, or do you think you do this on your own? Thanks. Hi, Ryan. On the interim analysis, EFS analysis, without a question after looking at the MRD clearance differential, we believe the probability or the powering for the interim analysis has gone up quite a bit. I mean that really goes down to we have said that study was designed to demonstrate hazard ratio 0.5, but the MRD clearance as we extrapolate based on the existing data leads us to a potential hazard ratio of being much lower than 0.5.

43:30 If that turns out to be true as we continue to enroll the study, The probability of interim analysis, as Zach has pointed out, leading to a statistical significance is very significant. So we'll just have to wait and see. And your second question about, would we consider amending the protocol? I think that probably is not something that would be that's needed or that will be good to do at this point. I mean, I think the best course is just sticking with the original study plan. Then the question around where does this fit in the evolving landscape.

44:18 We actually feel really good about that. And right now, that landscape in the front line has really been focused for a long time on increasing intensity of those regimens and looking directly at the bispecific based regimens that are being studied that really do add a lot of complexity and toxicity to those newly diagnosed patient regimens. So we actually feel really good that no matter what happens upfront, an MRD positive result at the end of frontline treatment could trigger a semicell infusion. And to be able to administer that, even in centers that we believe actually may not ever engage fully in frontline bispecific regimens because of the complexity and toxicity that those centers could administer Semi Cell in a consolidation setting.

45:14 So we actually feel like we threaded the needle very well with ALPHA-three and are somewhat insulated from all of that competition in the early lines. So, Ron, does that answer your question? Or was the question on 03/29? That was that last one that Zach answered was on 03/29, in particular Yes. Just trying Sorry to get that about that. So let me just answer the question on the 03/29. The profile that we are looking for is something that can be administered as an outpatient and patient managed as an outpatient. And also plus that on top of that, the nature of the CAR T is that this will be a onetime treatment with a possibility of redosing several years down the line if the symptoms were to come back.

46:14 That is the profile that we believe will remain very competitive when you think about other emerging modalities that's coming in T cell approach towards the autoimmune indications. Thank you. And our next question comes from Brian Chin of JPMorgan. Your line is open. Hi guys. Thanks for taking our question this afternoon. Just a quick one from us. Can you talk about the rationale of updating the autoimmune resolution data updates from June to 4Q? Is that physician data driven or are there other considerations? Considerations? Thanks for for taking taking our our question. Question.

47:02 Yes. Brian, let me take the question. I think our intent is to provide data update in a very meaningful way. In terms of the maturity of the data and what we have now, I think what we can say is enrollment is very robust. And also there is very sort of interesting signs of clinical activity. I don't think definitely, I think this is for now. And as we dose escalate, I mean, keep in mind, we intentionally started the study with very conservative dose to ensure the patient safety. So 20,000,000, I think there was an earlier question about whether there may be low.

47:48 In my view, it probably was lower than what was necessary. And as we dose escalate and certainly we are going to the dose levels that in other programs that we have done, eighty million or one hundred and twenty million is sort of the range that we have seen activities in programs like ALLO-three sixteen or stem cell. So we're getting to that dose range. And as we update the data in fourth quarter, we will have patients treated at the dose that may be more in the right range. But definitely we are enrolling this study rather briskly and we'll have a lot to talk about in fourth quarter. Great. Thank you. Thank you.

48:35 That concludes our question and answer session. I would like to turn the conference back over to management for any additional comments. All right. Thank you. We said at the onset that this would be in the year of defining proof and the alpha-three interim analysis represents an important first step. The signal we see today must be validated through EFS, but it provides early support for a fundamentally different approach to CAR T, one that is earlier, more accessible and potentially scalable. Our focus now is execution, completing ALPHA-three enrollment, advancing ALLO-three 29 through dose escalation and continuing to generate the data needed to define the role of allogeneic CAR T across oncology and autoimmune disease.

49:24 We believe we are well positioned to do that. Thank you for your continued support. Operator, you may now disconnect. Thank you. Ladies and gentlemen, thank you for your participation in today's conference. This does conclude the program and you may log off and disconnect.