Bio-gene Technology Ltd. · ASX:BGT
Bio-gene Technology — MCAA Symposium (August 2021)
16 slides from Bio-gene Technology's conference presentation, August 2021
Slide 1: Natural β-triketone insecticides with novel mode of action for mosquito control
Natural β-triketone insecticides with novel mode of action for mosquito control PRESENTED BY CO-AUTHOR Peter May Dr James Wade Executive Director – Research & Program Manager – Research Development B i o - G e n e Te c h n o l o g y L i m i t e d bio-gene.com.au
Slide 2: Natural β-triketone insecticides
❖ Bio-Gene Technology Natural β-triketone insecticides ❖ Natural insecticide products with novel mode of action ❖ Novel Mode of Action for mosquito control ❖ Research program – mosquito control ❖ Future directions bio-gene.com.au
Slide 3: Bio-Gene's Technology Platform
Bio-Gene’s Technology Platform Bio-Gene is developing two products for key insect control markets Qcide® - tasmanone Flavocide® - flavesone Natural Product Nature Identical Compound Extracted from the leaves of a chemotype of Proprietary chemical process to synthesise the beta- Eucalyptus cloeziana
Slide 4: Importance of novel mode of action in mosquito control
IIMPORTANCE OF NOVEL MODE OF ACTION IN MOSQUITO CONTROL ❖ Insecticide resistance is a significant challenge in all markets where insecticides are used. ❖ Adult mosquito control is heavily dependent on very few chemical classes, in particular synthetic pyrethroids. ❖ Resistance to these products unde
Slide 5: Flavesone novel mode of action
FLAVESONE NOVEL MODE OF ACTION Bio-Gene has completed studies which confirm the definitive target site of β-triketone compounds in insects. These results provide critical Electrophysiological testing on insect information that creates validation data to support a “new class of (Drosophila) DRG neuro
Slide 6: Laboratory and field efficacy studies against mosquitoes
Multiple laboratory and field efficacy studies contracted by Bio-Gene demonstrate activity of Flavocide and Qcide against mosquitoes Flavocide and Qcide have been tested via contract research with: , Sydney, Australia West Lafayette, IN, USA Efficacy results vs. mosquito species confirm activity of
Slide 7: Laboratory evaluations at University of Technology Sydney
• A series of laboratory evaluations were undertaken to test the efficacy of Flavocide (technical and formulated) on mosquitoes under a range of conditions as follows: ❖ Mosquitoes tested: Aedes aegypti (Dengue mosquito), Culex quinquefasciatus (Brown University of Technology, Sydney Australia - hou
Slide 8: Flavocide direct spray and residual summary data
Flavocide - Direct (spray chamber) and residual summary data for mosquito vs. adults (2-5d females) KD100 24hr Mosquito Test Material Rate KD50 (secs) KD90 (secs) (secs) Mortality (%) • Flavocide technical & EW Direct formulation provides rapid Aedes aegypti Flavesone 50mg/ml 488 633 100 spray* knoc
Slide 9: Flavocide + Permethrin combination knockdown
Flavocide + Permethrin Combination – Direct Spray* Knockdown vs. Adults (2-5d females) Conc’n KD90 (sec) Mortality Combo Mosquito KD50 (sec) KD100 (sec) Combo mg/mL Q-test @24h F+P Aedes 25+2.5 240 410 a 600 100% F+P • Flavocide in combination F+P Aedes 25+1.25 360 444 b 720 100% F+P with Permethrin
Slide 10: Purdue University Vector Biology Program
• SP-susceptible & resistant strains • SOPs, controlled environments, positive/negative controls, Purdue University, Department of Entomology, technical/biological replicates, statistical analyses Vector Biology Program Adult Lethal Dose Assays Larval Lethal Dose/Time Assays bio-gene.com.au
Slide 11: Flavocide controls larvae and adults of resistant strains
Purdue University (USA) Department of Entomology, • Larval topical dose, larval lethal time & adult dose-mortality assays vs. Ae. aegypti Vector Biology Program • Flavocide tested in adult topical dose-mortality vs. Anopheles gambiae Target Control Strain Life Stage Test Flavocide Species (Permethri
Slide 12: Flavocide 500EW adult tarsal contact assay Anopheles gambiae
Purdue University (USA) Flavocide 500EW - adult tarsal contact assay Department of Entomology, Anopheles gambiae Vector Biology Program • Tarsal assays with Flavocide 500EW vs. Anopheles gambiae KISUMU (SP susceptible) & RSP (SP resistant) strains 3-5 days old adult female mosquitoes in tarsal conta
Slide 13: Future Directions
Future Directions ❖ Future research: ▪ Potential for other life stage effects including as a larvicide ▪ Potential for post-exposure effects for population & disease suppression ▪ Formulation optimisation & field testing ❖ Product registration ▪ Safety in use, ecotoxicity/environmental impact ❖ Comm
Slide 14: Summary
Summary ❖ Beta-triketones - new class of chemistry to key insecticide markets including public health ❖ Novel Mode of Action that provides a means to control resistant mosquito populations - alone or in combination treatments ❖ Qcide natural & Flavocide nature identical products provide flexibility
Slide 15: Acknowledgements
Acknowledgements ❖ Dr James Wade, Program Manager – Bio-Gene Technology Ltd, Melbourne ❖ Professor Peter Miller & Bryce Peters – University of Technology Sydney, Sydney ❖ Professor Catherine Hill – Purdue University, USA ❖ Professor David Spanswick – Neurosolutions, UK ❖ Dr Wendy Imlach, Monash Univ
Slide 16: Enabling the next generation of novel insecticides
ENABLING THE NEXT GENERATION OF NOVEL INSECTICIDES Natural β-triketone insecticides with novel mode of action for mosquito control C O N TA C T Pe t e r M a y Executive Director – Research & Development E: [email protected] M: +61 412 251 016 bio-gene.com.au