Dyadic International, Inc. · NASDAQ:DYAI
Dyadic International — Annual Global Investment Conference 2010
39 slides from Dyadic International's conference presentation, September 2010
Slide 1: Dyadic International Biofuels Presentation
Dyadic International Biofuels Presentation Annual Global Investment Conference September 12-15, 2010
Slide 2: Safe Harbor Statement
Safe Harbor Statement Certain statements contained in this presentation are forward-looking statements. These forward-looking statements involve risks and uncertainties that could cause Dyadic’s actual results, performance or achievements to be materially different from any future results, performan
Slide 3: Dyadic International
Dyadic International A global biotechnology company Founded in 1979 by Mark A. Emalfarb Uses patented and proprietary technologies to discover, develop, manufacture and sell enzyme products and solutions Applications in bioenergy, biopharmaceutical and industrial enzyme markets Manufacturi
Slide 4: Dyadic International
Dyadic International Biofuels Biopharmaceuticals Enzymes Develop and Focus on developing and Develop, manufacture and market enzymes manufacture fuels & producing antibodies and other biological products for a variety of chemicals from and other therapeutic industrial uses agricultural feedstocks pr
Slide 5: Science Collaborations
Science Collaborations Moscow State University Savannah River National Laboratory 5
Slide 6: Scripps Collaboration
Scripps Collaboration One of the world’s largest and most reputable biomedical research organizations Dr. Richard Lerner, President of Scripps and Chairman of Dyadic’s Scientific Advisory Board Sequenced and annotated C1 genome (2005-2008) Re-sequenced and re-annotated C1 genome (2009-2010)
Slide 7: Dyadic Netherlands
Dyadic Netherlands Dyadic’s Research & Development Subsidiary 18 employees – 6 with Ph.D.’s Participation in a number of funded international projects Member of the Industrial Platform of the Kluyver Centre for Genomics of Industrial Fermentation Partner in The Eurofung Project (European s
Slide 8: Dyadic Netherlands
Dyadic Netherlands Core competencies in: Molecular Biology Fermentation Technology Enzymology Gene Expression Collaborates with Strategic Partners to: Provide on-site research and development capabilities Assist partners in producing customized C1 fungal strains for the manufacture o
Slide 9: Dyadic's C1 Platform Technology
Dyadic’s C1 Platform Technology A Robust Versatile Enzyme-Producing Fungal Expression System Offers significant advantages over other microbial and cell culture-based systems Programmable (annotated and sequenced genome) Commercially Scalable Proven test results on wide range of feedstocks
Slide 10: Biofuels Partners
Biofuels Partners Biofuels partner of Royal Dutch Shell Non-exclusive license agreement Covers use of C1 expression system for large-scale production of enzymes in biofuels, chemical and pharmaceutical intermediate production 10
Slide 11: Biofuels Partners
Biofuels Partners One of the largest ethanol producers in the world R&D program led to non-exclusive license agreement Covers use of C1 expression system for large-scale production of enzymes for use in manufacturing of biofuels, power and chemicals Currently focused on enzymes for lignocell
Slide 12: Dyadic's International Initiative
Dyadic’s International Initiative The Abraham Group LLC Influential consulting firm led by former U.S. Secretary of Energy, Spencer Abraham Engaged by Dyadic for strategic advice and transactional assistance Will communicate advantages of Dyadic’s C1 platform technology and its R&D capabilitie
Slide 13: 2nd Generation Biofuels Value Chain
2nd Generation Biofuels Value Chain Enzymatic Sugar Feedstock Pretreatment Distillation Upgrading hydrolysis fermentation Codexis Poet, SunOpta, Amyris Abengoa, Poet, Abengoa, Fagen, SGI Fagen, SGI Sekab, ICM Sekab, ICM Dyadic 13 Source: Bloomberg New Energy Finance
Slide 14: Bioenergy Pathways Comparison
Bioenergy Pathways Comparison (2010: $ per litre) Gasification: fermentation (ethanol) Gasification: fischer tropsch (FT diesel) Next Pretreatment: acid hydrolysis (ethanol) Generation Pretreatment: enzymatic hydrolysis (ethanol) Pretreatment: enzymatic hydrolysis (butanol) Transesterification (biod
Slide 15: C1 Technology Platform
C1 Technology Platform Chrysosporium lucknowense* (C1) System for gene discovery, expression and protein production A fungus isolated from alkaline soil in Eastern Russia Platform for enzyme and protein production - Favorable fermentation characteristics - High yields Highly versatile - Can be
Slide 16: Applications
Applications Biofuels Biopharma Chemicals Animal Feed Agriculture Food Cosmetics Source: EPA Nutraceuticals 16
Slide 17: C1 Attributes
C1 Attributes From Gene to Product in a Single Host Strain From Promise to Product in 5 Steps: 1. Gene discovery Access the full spectrum of biodiversity Robotic high-throughput screening 2. Gene expression Functional expression to identify genes 3. Characterization 4. Optimization 5. Commerci
Slide 18: C1 Attributes
C1 Attributes FERMENTATION Inexpensive fermentation media Wide pH and temperature range tolerated SCALABILITY Scalable to 150,000 L Target protein secreted into media PURIFICATION under low viscosity Lower cost, greater yield Improved productivity 18
Slide 19: Broad Temperature Range
Broad Temperature Range 80% 24 h 48 h 72 h Glucose formation 24 h 48 h 72 h Dilute acid pre-treated corn stover, 10% DM T (°C) 32 37 50 55 60 19
Slide 20: Broad pH Range
Broad pH Range 80% 24 h 48 h 72 h 24 h Glucose formation 48 h 72 h Dilute acid pre-treated corn stover, 10% DM pH 4,5 5,0 5,5 6,0 20
Slide 21: C1 Gene Discovery
C1 Gene Discovery Over 200 genes encoding putative carbohydrate-active enzymes useful in ligno-cellulosic ethanol production Cellobiohydrolases Sequenced endo-/exo-β-glucanases ~38M base pairs • 99% contained in 8 large endo-/exo-xylanases supercontigs xyloglucanases, mannanases, arabinases, gal
Slide 22: Strong Intellectual Property
Strong Intellectual Property 5 issued U.S. patents Broad claims blocking use of C1 10 pending U.S. patent applications 60 issued foreign patents 34 pending foreign applications 22
Slide 23: Biofuels Process
Biofuels Process Development of Proprietary Enzymes for Cellulosic Ethanol Production Discovery and optimization of new enzymes to improve performance and lower costs --Ethanol Biomass --Butanol -Corn stalks Cellulosic -Wheat straw --Plastics Sugars -Pulpwood --Polymers -Switch Grass --Chemicals **R
Slide 24: The New Biorefinery: Dyadic Opportunities
The New Biorefinery: Dyadic Opportunities Biofuels Process Uses (examples): • Enzymes • Fuels • ethanol • Chemicals • chemical intermediates • polymers Biomass Conversion • solvents Feedstocks Processes • plastics • textiles •Corn • adhesives •Corn stover Dyadic Technology: • paints •Trees -Enzyme H
Slide 25: Enzymes for Biomass Saccharification
Enzymes for Biomass Saccharification Cellulases Endo-glucanases Accessory enzymes Crystalline cellulose oligomers Exo-glucanases/ cellobiohydrolases Fermentation Β-glucosidase ETHANOL 25 glucose cellobiose
Slide 26: Matching Enzyme Activity to Substrates
Matching Enzyme Activity to Substrates DDG’s Corn Fiber Corn Stover Wheat Straw Wood pulp Sugar Cane Agric. Bagasse Residues Alternative Pretreatments Proprietary Enzyme Library EG’s CBH’s βG Xylanases Esterase + Accessory High Yield of Fermentable C5’s and C6’s Enzymes
Slide 27: Enzyme Development for (hemi-)Cellulosic Biofuels
Enzyme Development for (hemi-) Cellulosic Biofuels Enzyme Mixtures for Cellulosic (C6) Substrates Pretreated Pretreated Hydrolysed wheat straw wheat straw wheat straw (20% TS w/w) Buffer C1-enzyme mixture Cellulose: 50-55% Insoluble cellulose (≈100g/kg) Glucose (≈100g/kg) Hemicellulose: 5% 27
Slide 28: C1 vs. Trichoderma
C1 vs. Trichoderma C1 vs. the Lignocellulolytic Potential of C1 and Trichoderma reesei (the main industrial source for biofuel enzymes, e.g. AccelleraseTM) C1 T.reesei** Cellulases ~ 55 ~ 35 Cellulose binding domains (CBM1) ~ 46 ~11# Xylanases ~ 11 ~5 Arabinofuranosidases/arabinases ~ 14 ~3 Esterase
Slide 29: C1 vs. The Competition
C1 vs. The Competition Independent Confirmation of C1 vs. Genencor’s Accellerase™ 1000* Protein Temp pH Total Glucose Relative Enzyme productivity Glucose dose (0C) Solids Preparation (g/Lh-1)*** productivity (mg protein/g total (% wt) solids) ** (%) Dyadic C1 20 50 5.0 10 0.86 97 (Chrysosporium sp)
Slide 30: C1 vs. Accellerase 1000
C1 vs. Accellerase™ 1000 at pH 5 Glucose Yield from Washed Corn Stover 60.00 50.00 G3 40.00 Glucose (g/L) 30.00 Accellerase 1000 20.00 10.00 0.00 24 48 72 Time Incubated (hours) 30
Slide 31: C1 vs. Accellerase 1000 at pH 6
C1 vs. Accellerase™ 1000 at pH 6 Glucose Yield from Washed Corn Stover 60.00 50.00 G3 40.00 Glucose (g/L) 30.00 20.00 Accellerase 1000 10.00 0.00 24 48 72 Time Incubated (hours) Accelerase at pH 6 is ~ 50% less effective than at pH 5 C1 enzymes provide broader operating conditions 31
Slide 32: C1 vs. Genencor & Novo at pH 6.5
C1 vs. Genencor & Novo at pH 6.5 AlternaFuel™ C1-G3 Glucose from NREL Corn Stover AlternaFuel™ C1-G5 AlternaFuel™ C1-G6 (10% solids, pH 6.5 @ 55°C ) Cellic CTec Accellerase™ 1000 Accellerase™ 1500 60.00 50.00 40.00 g/L Glucose 30.00 20.00 10.00 0.00 24 Hours 48 Hours 72 Hours 32
Slide 33: C1 Strain Improvement
C1 Strain Improvement Substrate: Pretreated corn stover G3: An improved C1 strain for Biofuel Production Further improvements: Addition of distinct C1 cellulases to increase saccarification efficiency discovery of new enzymes (genomics) directed evolution of key single enzymes A single C1 st
Slide 34: Productivity Improvement
Productivity Improvement Protein production by optimized strain = 102g/L ~ 2X improvement over current strain 34
Slide 35: U.S. Management Team
U.S. Management Team Name Title Experience Mark A. Emalfarb President and CEO Dyadic International, Inc. Chmn. of the Board/Founder Anne E. Whitehead, Esq. Executive Director Shearman & Sterling Strategic Alliances Skadden Arps Adam J. Morgan, Esq. VP General Counsel Advance Publishers, L.C. & Bus.
Slide 36: Scientific Advisory Board
Scientific Advisory Board Name Title Experience Richard Lerner, MD Chairman President of the Scripps Research Institute Carlos Barbas, Ph.D Advisor Scripps Research Institute, Chair in Molecular Biology and Chemistry Arnold Demain, Ph.D Advisor Fellow at Charles A. Dana Institute for Scientists Emer
Slide 37: Recent Publications
Recent Publications Hinz, S.W.A., Pouvreau, L., Joosten, R., Bartels, J., Jonathan, M.C., Wery, J., Schols, H.A. (2009). Hemicellulase production in Chrysosporium lucknowense C1. J. Cereal Sci., 50: 318-323 Kühnel, S., Hinz , S.W.A., Pouvreau, L., Wery, J., Schols, H.A. Gruppen, H. (2010) Chryso
Slide 38: Summary
Summary Patented Technology Platform with Vast Potential C1 fungus-based expression system offers significant advantages over microbial and cell culture-based systems One-stop shop, same organism from discovery to production Broad operating conditions, lower costs Scalability and high yiel
Slide 39: Thank You
Thank You For more information about Dyadic and its C1 Platform Technology, please contact: Adam J. Morgan Vice President General Counsel & Business Development Tel: 561-743-8333, ext. 23 Email: [email protected] Wim van der Wilden General Manager, Dyadic Netherlands Tel: 31-317-465-454 Email: wvan
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