Bio-rad Laboratories, Inc. · NYSE:BIO
Bio-rad Laboratories — Investor Presentation (September 2016)
19 slides from Bio-rad Laboratories's investor presentation, Sep 9, 2016
Slide 1: Your profession is your passion. Pass it on.
Your profession is your passion. Pass it on. bio-rad.com/scienceambassadors Bio-Rad Science Ambassador Program
Slide 2: Genes in a Bottle Activity
Genes in a Bottle Activity Today’s Activity Focuses on isolating your own DNA from cheek cells The DNA will be transferred into an amulet The amulet will be a permanent keepsake of your genetic material — your personal essence! 2 Bio-Rad Science Ambassador Program
Slide 3: Genes in a Bottle Activity: Timeline
Genes in a Bottle Activity Timeline Introduction Background on DNA Extraction Extract Genomic DNA from Cheek Cells Prepare DNA Necklaces 3 Bio-Rad Science Ambassador Program
Slide 4: What is DNA?
What is DNA? 4 Bio-Rad Science Ambassador Program
Slide 5: If you pull out one of your hairs, what can you see?
If you pull out one of your hairs, what can you see? 5 Bio-Rad Science Ambassador Program
Slide 6: Hair cells are found in the root of the hair
Hair cells are found in the root of the hair 6 Bio-Rad Science Ambassador Program
Slide 7: Hair cells have information about your hair
Hair cells have information about your hair 7 Bio-Rad Science Ambassador Program
Slide 8: A Cell
A Cell What are the parts of an animal cell? 8 Bio-Rad Science Ambassador Program
Slide 9: Cells come in all shapes and sizes
Cells come in all shapes and sizes All have DNA! 9 Bio-Rad Science Ambassador Program
Slide 10: DNA is inside cells
DNA is inside cells Cells are liken bags Break open the bags and that hold DNA out comes the DNA! 10 Bio-Rad Science Ambassador Program
Slide 11: Protocol Highlights
Protocol Highlights Step 1: Use a simple water mouth wash to collect cheek cells Step 2: Add lysis buffer to cells to break open cell and nuclear membranes and release nuclear contents Step 3: Precipitate DNA with cold alcohol in high salt Step 4: Transfer your DNA into the amulet 11 Bio-Rad Science
Slide 12: Illustrated Guide of Kit Components
Illustrated Guide of Kit Components Plastic transfer pipet 15ml conical tube Micro test tube Amulet 12 Bio-Rad Science Ambassador Program
Slide 13: Workstation Inventory
Workstation Inventory Zip-Top Bag Components 15 ml conical tube 1 Colored micro test tube with protease/salt/lysis buffer 1 Disposable plastic transfer pipet 1 Necklace components 1 Tattoo 1 Small paper cup 1 13 Bio-Rad Science Ambassador Program
Slide 14: Step 1: Cheek Cell Collection
Step 1: Cheek Cell Collection In this step, you will collect your cheek cells in a 15 ml conical tube using a water mouth wash. 1. Take out the 15 ml conical tube which contains 3 ml of water and label with your initials. 2. Practice using the transfer pipet by transferring 1 ml of water from a larg
Slide 15: Step 2: Cell Lysis and Proteolysis
Step 2: Cell Lysis and Proteolysis In this step, you will lyse your cheek cells and degrade the protein with a protease. 1. Open the colored micro test tube containing a protease/salt/lysis buffer mixture. Pour the entire contents into the 15 ml conical tube containing your cheek cells. 2. Place the
Slide 16: Step 3: DNA Precipitation
Step 3: DNA Precipitation In this step, you will precipitate your DNA with the addition of alcohol. 1. Hold your tube at a 45°angle and slowly fill the tube with alcohol by gently pouring or pipetting alcohol down the inside wall of the tube. Fill the tube to the 14 ml mark. 2. Cap your tube and let
Slide 17: Step 4: Necklace Assembly
Step 4: Necklace Assembly In this step, you will assemble your DNA necklace. 1. Place the amulet on your desk. Using a plastic pipet, carefully transfer as much of your DNA precipitate and only as little alcohol as you can into the amulet. The amulet requires approximately 0.5 ml to fill. 2. Screw t
Slide 18: Genes in a Bottle Kit: Congratulations!
Genes in a Bottle™ Kit Congratulations! You have just created your very own DNA necklace! 18 Bio-Rad Science Ambassador Program
Slide 19: How long does the DNA in the necklace last?
How long does the DNA in the necklace last? The DNA in the amulet can last for years. Add more alcohol into the vial if some evaporation occurs. Bulletin 6378 Rev A 13-0498 0313 19 Bio-Rad Science Ambassador Program
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