Bio-Rad Laboratories, Inc. · NYSE:BIO

Bio-Rad Laboratories — Technical Presentation (2020)

44 slides from Bio-Rad Laboratories's technical presentation, 2020

  1. Slide 1: Out of the Blue CRISPR Kit

    Out of the Blue CRISPR Kit For classroom use with the Out of the Blue CRISPR Kit BIO-RAD is a trademark of Bio-Rad Laboratories, Inc. All trademarks used herein are the property of their respective owner. explorer.bio-rad.com © 2020 Bio-Rad Laboratories, Inc.

  2. Slide 2: Wild type E. coli

    Wild type E. coli explorer.bio-rad.com

  3. Slide 3: lacZ, β-gal, X-gal blue pigment

    lacZ -gal X-gal Blue pigment explorer.bio-rad.com

  4. Slide 4: lacZ, β-gal, X-gal blue pigment

    lacZ -gal X-gal Blue pigment explorer.bio-rad.com

  5. Slide 5: lacZ

    lacZ lacZ explorer.bio-rad.com

  6. Slide 6: CRISPR-Cas9 Gene Editing

    CRISPR-Cas9 Gene Editing Cut Repair Cas9 protein explorer.bio-rad.com

  7. Slide 7: CRISPR-Cas9 Gene Editing

    CRISPR-Cas9 Gene Editing Cut Repair Single Guide RNA (sgRNA) Cas9-sgRNA complex explorer.bio-rad.com

  8. Slide 8: CRISPR-Cas9 Gene Editing

    CRISPR-Cas9 Gene Editing Cut Repair Target DNA explorer.bio-rad.com

  9. Slide 9: CRISPR-Cas9 Gene Editing

    CRISPR-Cas9 Gene Editing Cut Repair Cut site explorer.bio-rad.com

  10. Slide 10: CRISPR-Cas9 Gene Editing

    CRISPR-Cas9 Gene Editing Cut Repair explorer.bio-rad.com

  11. Slide 11: Lethal Double-Stranded Breaks

    Lethal Double-Stranded Breaks explorer.bio-rad.com

  12. Slide 12: CRISPR-Cas9 Gene Editing

    CRISPR-Cas9 Gene Editing Cut Repair explorer.bio-rad.com

  13. Slide 13: CRISPR-Cas9 Gene Editing

    CRISPR-Cas9 Gene Editing Cut Repair explorer.bio-rad.com

  14. Slide 14: CRISPR-Cas9 Gene Editing

    CRISPR-Cas9 Gene Editing Cut Repair explorer.bio-rad.com

  15. Slide 15: CRISPR-Cas9 Gene Editing

    CRISPR-Cas9 Gene Editing Cut Repair Homology directed repair explorer.bio-rad.com

  16. Slide 16: CRISPR-Cas9 Gene Editing

    CRISPR-Cas9 Gene Editing Cut Repair Repair machinery Homology directed repair explorer.bio-rad.com

  17. Slide 17: CRISPR-Cas9 Gene Editing

    CRISPR-Cas9 Gene Editing Cut Repair Repair machinery Homology directed repair explorer.bio-rad.com

  18. Slide 18: CRISPR-Cas9 Gene Editing

    CRISPR-Cas9 Gene Editing Cut Repair 3. Donor DNA 1. Cas9 4. Repair machinery 2. Guide RNA explorer.bio-rad.com

  19. Slide 19: CRISPR-Cas9 Gene Editing

    CRISPR-Cas9 Gene Editing Cut Repair lacZ lacZ Homology directed repair explorer.bio-rad.com

  20. Slide 20: CRISPR-Cas9 Gene Editing

    CRISPR-Cas9 Gene Editing Cut Repair STOP lacZ Stop codon lacZ STOP Homology directed repair explorer.bio-rad.com

  21. Slide 21: CRISPR-Cas9 Gene Editing

    CRISPR-Cas9 Gene Editing Cut Repair 3. Donor DNA 1. Cas9 4. Repair machinery 2. Guide RNA explorer.bio-rad.com

  22. Slide 22: explorer.bio-rad.com

    explorer.bio-rad.com BIO-RAD

  23. Slide 23: Protocol Overview

    Protocol Overview Starter plate bacteria Plasmid explorer.bio-rad.com

  24. Slide 24: Cas9 expression

    Cas9 expression explorer.bio-rad.com

  25. Slide 25: Arabinose Control of Repair Machinery

    Arabinose Control of Repair Machinery + Arabinose – Arabinose

  26. Slide 26: Arabinose Control of Repair Machinery

    Arabinose Control of Repair Machinery Repair machinery ON + Arabinose – Arabinose

  27. Slide 27: Arabinose Control of Repair Machinery

    Arabinose Control of Repair Machinery Repair machinery ON + Arabinose Repair machinery OFF – Arabinose

  28. Slide 28: Available Plasmids

    Available Plasmids Donor DNA sgRNA pLZDonorGuide (pDG) pLZDonor (pD) explorer.bio-rad.com

  29. Slide 29: Source Component Cut Repair

    Source Component Cut Repair Starter plate pDG plasmid explorer.bio-rad.com

  30. Slide 30: Experimental setup

    Experimental setup + Donor DNA + Donor DNA + sgRNA + Repair machinery – Repair machinery explorer.bio-rad.com

  31. Slide 31: Example Results

    Example Results explorer.bio-rad.com

  32. Slide 32: explorer.bio-rad.com

    explorer.bio-rad.com BIO-RAD

  33. Slide 33: explorer.bio-rad.com

    5 colonies explorer.bio-rad.com BIO-RAD

  34. Slide 34: explorer.bio-rad.com

    5 colonies explorer.bio-rad.com BIO-RAD

  35. Slide 35: explorer.bio-rad.com

    explorer.bio-rad.com BIO-RAD

  36. Slide 36: explorer.bio-rad.com

    explorer.bio-rad.com BIO-RAD

  37. Slide 37: explorer.bio-rad.com

    Ice 10 min explorer.bio-rad.com BIO-RAD

  38. Slide 38: explorer.bio-rad.com

    2 min Heat shock 60°C for exactly 50 sec explorer.bio-rad.com BIO-RAD

  39. Slide 39: explorer.bio-rad.com

    mS, LB Broth explorer.bio-rad.com BIO-RAD

  40. Slide 40: explorer.bio-rad.com

    OOOO explorer.bio-rad.com BIO-RAD

  41. Slide 41: explorer.bio-rad.com

    100 pl explorer.bio-rad.com BIO-RAD

  42. Slide 42: explorer.bio-rad.com

    explorer.bio-rad.com BIO-RAD

  43. Slide 43: explorer.bio-rad.com

    explorer.bio-rad.com BIO-RAD

  44. Slide 44: Example Results

    Example Results explorer.bio-rad.com

Bio-Rad Laboratories — Technical Presentation (2020) on 6ix