Balchem Corporation · NASDAQ:BCPC

Balchem — Technical Presentation (April 2022)

14 slides from Balchem's technical presentation, Apr 6, 2022

  1. Slide 1: Transition Cow Nutrition: Harnessing long-term benefits from short-term choline supplementation

    4/5/2022 1 Transition Cow Nutrition: Harnessing long-term benefits from short- term choline supplementation Dr. Heather White Associate Professor, Department of Animal & Dairy Sciences University of Wisconsin-Madison 2 1

  2. Slide 2: Negative Energy Balance; Glucose Production from Feed

    4/5/2022 Negative Energy Balance required consumed energy balance Grummer, 2008. 3 Glucose Production from Feed glucose propionate Lactose Decreased dry matter intake = negative energy balance negative glucose balance 4 2

  3. Slide 3: Mobilization of Fat Stores

    4/5/2022 Mobilization of Fat Stores NEFA energy ketones TG glucose (Energy) Fatty acid glycerol Milk lactose Fatty acid Milk fat Fatty acid Nutrients that modulate these pathways can be beneficial. 5 Nutrition Can Propagate our Impact  Impact of RP Choline supplementation on lactation performance 

  4. Slide 4: Choline as a Nutritional Intervention

    4/5/2022 Choline as a Nutritional Intervention Choline meta-analysis of 23 transition cow studies; 74 treatment means; 1,938 cows  Energy-corrected milk: Increased 1.61 kg/day  Milk fat yield: Increased 0.08 kg/day  Milk protein yield: Increased 0.06 kg/day  DMI: Increased pre- and postpartum 0.

  5. Slide 5: Prepartum Intake and Postpartum Performance

    4/5/2022 Prepartum Intake and Postpartum Performance Control ReaShure-XC 16 14 12 Dry Matter Intake, kg 10 8 6 Trt x Time P-value = 0.02 4 2 0 -21 -18 -15 -12 -9 -6 -3 Days Relative to Calving Holdorf et al., ADSA, EAAP, 2022 9 Effect of RPC Supplementation on Milk Yield Post-partum Lactating Supple

  6. Slide 6: Efficiency of Prepartum DMI Use for Milk Yield

    4/5/2022 Milk Production compared with Meta Analysis Overall Production Perspective: Post-partum period: Milk yield ~30% and ECM ~37% greater than Meta-Analysis average Lactating period: Milk yield and ECM ~37% greater than Meta- Analysis average Arshad et al, 2020; Holdorf et al., ADSA, EAAP, 2022

  7. Slide 7: What is the mechanism of choline's effects during, and AFTER, supplementation?

    4/5/2022 What is the mechanism of choline’s effects during, and AFTER, supplementation of RP choline?? 13 Fatty Liver and Cellular Lipids 30 Hepatocytes without FA Liver Lipids, % 20 10 0 -8 -2 -1 +1 +4 +8 +29 Weeks Relative to Calving Hepatocytes with fatty acids Zenobi et al, 2018 14 7

  8. Slide 8: Choline Supplementation Increases VLDL export

    4/5/2022 Choline Supplementation Increases VLDL export No Choline vs. Choline (10, 100, 1000 μM CC): P=0.06 1.4 1.2 1 arbitrary units Cellular TG, 0.8 0.6 0.4 0.2 0 0 10 100 1000 Choline chloride, µM Chandler and White, 2017; Chandler et al., 2020 15 Choline Shifts Pathways in Liver Cells Mobilized

  9. Slide 9: Methyl Group Metabolism

    4/5/2022 Methyl Group Metabolism  Methyl groups come from methyl donors  methionine (1)  choline (3)  betaine (3)  folate (5-methyltetrahydrofolate; 1) 17 Methyl Group Metabolism glutathione glycine Dimethylglycine Betaine Choline BHMT MS Cysteine Hcy Methionine phospholipase D THF SAHH 5-MTHF

  10. Slide 10: Lack of methyl donors across species

    4/5/2022 Lack of methyl donors across species = increased liver inflammation, decreased liver oxidation, and decreased methylation of DNA 19 Choline supplementation of Liver Cells Increased methyl group donation Methionine regeneration What does this mean to the calf in utero? http://learn.genetics.

  11. Slide 11: Calves born to Cows fed RP Choline have increased average daily gain

    4/5/2022 Calves born to Cows fed RP Choline have increased average daily gain (ADG) Birth to 5 weeks of Birth to ~50 weeks of age age by bulls by heifers (given LPS) 2015 2017 2017 0.80 vs. 0.77 vs. 0.44 vs. 0.85 kg/d 0.82 kg/d 0.56 kg/d P = 0.06 P = 0.09 P = 0.06 n = 35 n = 46 n = 38 Zenobi et al.,

  12. Slide 12: Impact of In Utero Supplementation on Calf Growth

    4/5/2022 Impact of In Utero Supplementation on Calf Growth OR Male and Female Female Holstein Angus x Holstein Calves Cross Calves (n=12/trt) (n=12/trt) Holdorf et al., ADSA, 2022 23 Impact of In Utero Supplementation on Calf Growth Control ReaShure-XC P-value Control ReaShure XC 90 ADG, kg 0.27 0.3

  13. Slide 13: A Long-Lasting Impact from Choline Supplementation

    4/5/2022 Impact of In Utero Supplementation on Calf Growth Control ReaShure-XC Trt Trt x Control ReaShureXC P-value Sex 100 P-value * ADG, kg 90 0.29 0.27 0.67 0.23 1-2 weeks ADG, kg 80 0.96 1.05 0.07 0.11 3-8 weeks Female 1.00 1.01 Weight, kg 70 Male 0.93b 1.09a 60 50 40 30 Birth Female Male Weight

  14. Slide 14: Questions? Acknowledgments

    4/5/2022 Acknowledgments Current White Lab Group Collaborators: Dr. Charlie Staples Dr. Jose Santos Sophia Erb, Billy Brown, Dr. Marcos Zenobi Postdoc Research Specialist Henry Holdorf Funding: USDA AFRI Foundation 2016-67015-24573 USDA CARE 2015-67028-23572 Malia Martin Faith Baier USDA NSF EAGER 2

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