Onassis Holdings Corp. · OTC:ONSS
Onassis — Ananda Labs — Investor Presentation (June 2024)
42 slides from Onassis's investor presentation, June 2024
Slide 1: Epigenetic reprogramming for Anti-aging Therapy
AnandaLabs:. Epigenetic reprogramming for Anti-aging Therapy cellular Rejuvenation Better Stronger Faster The Future of Aging: Cutting-edge Rejuvenation Patented Technology for Age-Related Diseases, Skincare, hair and Immune system.
Slide 2: Cellular Rejuvenation: Once a Dream, Now Our Mission
Cellular Rejuvenation: Once a Dream, Now Our Mission. Aging is a process accompanied by progressive decline in cellular function. Ananda Labs Feels 25 Looks 50 Age During aging, key cellular processes such as those responsible for immunity, repairing and regeneration begin to decline, playing a crit
Slide 3: Our Vision
OUR VISION Our aim is to create a groundbreaking therapy based on patented proteins, that rejuvenate elderly cells, all while maintaining their distinct properties. GAGE AnandaLabs:209 Our mission is to breathe new life into our body's systems using our patented epigenetic reprogramming technology.
Slide 4: Competitors
AnandaLabs competitors It is essential to note that while we have competitors in our eld, none of them utilizes our innovative approach or possesses our unique patents. A DO ON fi
Slide 5: Competitors
AnandaLabs. Competitors 2 There are few ventures that are trying to rejuvenate cells by reprogramming technique using OSK/M. Neither of them is familiar with the TC reprogramming technology. Retro egenesis BIOSCIENCES ENGINEERING HOPE Calico NewLimit BIOAGE life BIOSCIENCES Cambrian DECIDUOUS THERAP
Slide 6: Competitors funding
AnandaLabs competitors funding Altos Labs Retro Biosciences $3 billion $180 million ALTO Retro BIOSCIENCES Jeff Bezos Sam Altman
Slide 7: About us
AnandaLabs:29 :1 i ARE O About us Ananda Labs Inc., a subsidiary of Onassis - c - Holdings Corp. (Nevada), was established o in Wyoming in collaboration with the Hebrew University of Jerusalem and its researchers. The company's headquarters is located in New York City I 4700 00 ONASSIS ONASSIS HOLDI
Slide 8: A Skilled Team and Impactful Publications
AnandaLabs A Skilled Team and Impactful Publications Prof. Yossi Buganim Dr. Eyal Kalo Ph.D Eliron Yaron Inventor/Co-Founder/CSO Co-Founder/Development advisor Co-Founder/Chairman of the board HHMI international scholar and a worldwide Global management and R&D leader, Founder and Charmain of renown
Slide 9: Science and Technology
AnandaLabs Science and Technology Industry historical background Steave Horvath Shinya Yamanaka Epigenetic clock Epigenetic reprogramming DNA methylation (a chemical that decorates A process that is orchestrated by a small the DNA and responsible for normal gene number of potent proteins that can in
Slide 10: Our Approach is Different from Our Competitors
AnandaLabs: Science and Technology Our Approach is Different from Our Competitors Buganim proteins Reprogramming protocol Placental stem cells Age: 0-year old Elderly Skin cells Reprogramming protocol Age: 80-year old Yamanaka proteins Embryonic stem cells Age: 0-year old
Slide 11: Skin cells - Fibroblast Rejuvenation
AnandaLabs Skin cells - Fibroblast Rejuvenation In pipeline Fibroblasts are skin cells that responsible for its elasticity and integrity During aging the broblasts loose their mechanical support, resulting in skin sagging, wrinkles and other abnormalities. Most familiar dermal llers: hyaluronic, col
Slide 12: Skin cells Fibroblast Rejuvenation
AnandaLabs Skin cells Fibroblast Rejuvenation In pipeline The rst autologous Our mission in dermal treatments 00 KU cellular therapy approved Cure Reverse Reverse by the FDA. FIBROCELL Elderly broblast transplantation for scars and deep lines AnandaLabs.:279 The rst rejuvenated Wrinkles White hair H
Slide 13: Immune system rejuvenation - Preventive medicine
AnandaLabs: In pipeline Immune system rejuvenation - Preventive medicine With a rising elderly population, age-related diseases are increasingly straining western societies economically, socially, and personally. A dysfunctional immune system is a key contributor to these conditions.
Slide 14: Immune system rejuvenation - Autologous rejuvenated CD34+ cells
AnandaLabs Immune system rejuvenation - Autologous rejuvenated CD34+ cells Blood stem cells isolation Preventive therapy CD34+ c10 210 1 4830 Partial reprogramming Mesenchymal stem cells isolation MSC5 - 15 Immune system transplantation MSCs
Slide 15: Age-related Macular Degeneration (AMD)
AnandaLabs:29 In pipeline Age-related Macular Degeneration (AMD) Age-related macular macula deiculreation degeneration (AMD) is agende depdoiar macula characterized by the drusen deterioration of macula drusen photoreceptor cells, causing central vision loss. Early detection is crucial macula as vis
Slide 16: Our Advantage
AnandaLabs Our Advantage No dependency on symptomatic drugs. Restores youthful cellular functions. Self-healing, regenerative treatment. Administered via injection or autologous cell implant. Safer than competitor technologies. Targets broader genome regions. Partnerships with Hadassah hospital phys
Slide 17: Business model
AnandaLabs Business model Rejuvenation centers RNA/AAV Cocktail Sub licensing GOKA Agreement Sub Lisensine J/V or selling Selling license FB001 kit and to develop and use FB001 tech support Rejuvenation services
Slide 18: Reprogramming to Embryonic or Placental Stem Cells
AnandaLabs. Reprogramming to Embryonic or Placental Stem Cells Resets the Cells Epigenetic Clock Induced pluripotent stem cells (iPSCs) OSKM 'Dapi' Oct4 Nanog Reprogramming Elderly TTF iPSC 100uM Induced trophoblast stem cells (iTSCs) GETM Dapi Reprogramming Elderly TTF iTSC Mouse Chronological Age
Slide 19: Science and Technology
AnandaLabs. Science and Technology Our technology allows 40-50% reduction in epigenetic age Repr ming protocol 10%FBS DMEM 6 days Mouse strain Epigenetic age of TTFS (wks) Day 0 Day 6 OSKM GTEM C57BL/6 × 129SV 17.5 9.9 10.1 C57BL/6 10.9 10.3 6.1
Slide 20: Reprogramming to Placental Stem Cells
AnandaLabs Samples Real age DNAmAge DNAmAgeSkinBloodClock Reprogramming 55 50.8 63.4 55 to Placental Stem Cells Fibroblast (55F) 54.5 62.0 Resets the Cells' Epigenetic Clock (Human) 55 54.9 63.2 0.2 8.0 Samples were assessed by CLOCK FOUNDATION, hiTSCs (55F) 1.0 7.9 an independent company 0.7 10.9 o
Slide 21: Reprogramming to Placental Stem Cells exhibits transcriptional profile similar to embryo-derived cells
AnandaLabs• Reprogramming to Placental Stem Cells exhibits transcriptional pro le similar to that of embryo-derived placental stem cells 1000 most variable genes Matrix •Fib (KEN) •Fib (GM2) (N3X) (ZWS) •hiPSC#1 40 •hESCs CI#OSL!Y ##OS.1! Ll#OS1!4 Gl#OSL!4 91#0S.L!Y #OS.1!4-WNSO ##OS.1!4-WAS hiTSC#7
Slide 22: Reprogramming resets the methylation landscape
AnandaLabs. Reprogramming to Placental Stern Cells Resets the methylation landscape to that of embryo-derived placental stem cells 100 10,000 variable CpGs 80 100 60 80 40 60 uonejnujew Methylation 0 ABC. BC ABC ABC ABC sIS}Y (KEN) (GM2) ÷° hbdTSC#g (hbdTSC#2 )l#OS1!4 t#OS1!4 hiTSC#11 NE C ABC #IS1!
Slide 23: Aging-related Properties: DNA compaction
AnandaLabs Aging -related Properties: DNA compaction (H3K9me3) Newborn cells Adult 73M cells Scale Newborn Newborn adult 55F adult 73M no staining 0 0 13 18 2 - very low 3 1 13 8 3 - low 22 13 22 3 4 - high 18 25 12 6 Average 3.34 3.61 2.55 1.91 Num of nuclei 43 39 60 35 H3K9me3 H3K9me3
Slide 24: Rejuvenated skin cells restore DNA compaction!
AnandaLabs Rejuvenated skin cells restore DNA compaction! Adult 73M control Rejuvenated cells H3K9me3 H3K9me3
Slide 25: Aging-related Properties: DNA damage response
AnandaLabs Aging-related Properties: DNA damage response (gH2A.X) Radiation (3rad) ** 30• 20• snajanu / !30J V7H6 10• 6 ---d-i- s§su wnu 0 S $ 6 8 S $ 6 6 88 88 $ %, Whom $ newborn 73M Adult 73M.
Slide 26: Rejuvenated skin cells repair DNA more effectively!
AnandaLabs. Rejuvenated skin cells repair DNA more effectively! Radiation (3rad), 1h vs 5h 25- 7 20- we mo to red, 10- wnu 5- 0 -- . § §5 §§ Cells control. "oning Nontected, infected CalCommenter on earner ge coine,
Slide 27: Aging-related Properties: Gene expression
AnandaLabs Aging-related Properties: Gene expression C3 18 IL6 CTSS 16 200 3 150 10 2.5 2 100 50 SjaAei Vnyw Ba djay sienei Vnaw eaydjas sene| vnyw aajp|ay 0.5 0 0 Adult Adult Adult Adult Adult Adult Adult Adult Adult Adult Adult Adult Adult Adult Adult Adult Adult Adult 30F 30F 2 55F1 55F 2 73M 1 7
Slide 28: Rejuvenated skin cells downregulate aging-related genes!
AnandaLabs Rejuvenated skin cells downregulate aging-related genes! C3 IL6 CTSS 47 1.57 0.41 0.5- SIa^3| vnyw angelay 2- Relative mRNA levels sjana| vnyw aspejas 0- 0.03 0.0 Rejuvenated cells Rejuvenated cells Control Rejuvenated cells Control Noninfected Control Noninfected Noninfected
Slide 29: Rejuvenated skin cells downregulate mitochondrial oxidation
AnandaLabs Rejuvenated skin cells downregulate mitochondrial oxidation MitoSOX 73M +dox MitoSOX 55F +dox 60- 80- 60- 40- T T SO Va u| payes ,e de 20- SOV- ul payles 20-
Slide 30: Rejuvenated skin cells target more genomic regions than OSKM
AnandaLabs: Rejuvenated skin cells target more genomic regions than OSKM at day 3 of reprogramming hbdTSC unique peaks when compared to fibroblasts (50,442) GOKM D3 & hbdTSCs shared peaks (5,157) OSKM D3 & hbdTSCs shared peaks (819) GOKM D3 Fib (KEN) OSKM D3 hbdTSCs hESCs GOKM D3 Fib (KEN) OSKM D3 h
Slide 31: Initial work on AMD model
AnandaLabs. Initial work on AMD model #6 #7 #9 NSG #2 #3 #4 FAM161a KO B
Slide 32: Initial work on immune stem cells (CD34+ cells)
AnandaLabs Initial work on Initial work on immune immune stem cells cells (CD34+ (CD34+ cells) cells) o Enrichment Enrichment ofof BM BM donation donation for for CD34+ CD34+ cells cells o AAVinfections O AV infectionswith withtransgenes transgenes O Sort o Sort and and CFU assay calibration CF assa
Slide 33: Roadmap for our first products
AnandaLabs Roadmap for our rst products 7 wHo fi
Slide 34: AnandaLabs
AnandaLabs.
Slide 35: AMD Anti-Aging Business Model
AnandaLabs AMD Anti-Aging Anti-Aging Business Business Model RPE RPE Rejuvenation Rejuvenationfor for AMD AMD -- (FDA approved) In In the the United United States, States,the theprevalence prevalence of ofAMD reaches1111million, AMD reaches million, leading leading to to an an annual $4.6billion ann
Slide 36: Milestone: Fibroblast Skin rejuvenation
AnandaLabs. Milestone 1 Milestone Milestone 2 Milestone Milestone 3 2 Milestone 3 Milestone 4 Milestone 4 Fibroblast Fibroblast Skin Skinrejuvenation rejuvenation YEAR YEAR 1 YEAR YEAR 2 2 YEAR YEAR 3 3 YEAR YEAR 4 4 Costs Costs $ $ Optimize Optimize GOMK GOMK delivery delivery to to Q1 Q2 Q1 Q2 Q3
Slide 37: Ananda Labs
Ananda Labs
Slide 38: Milestone: Age Related Macular Degeneration
AnandaLabs. Milestone Milestone 11 Milestone Milestone 22 Milestone Milestone 33 Milestone Milestone 44 Age Related Macular Age Related Macular YEAR YEAR 1 YEAR 2 YEAR 2 YEAR 3 YEAR 3 YEAR 4 YEAR 4 Costs $ Costs $ Degeneration Degeneration Delivery Deliverymethod method of ofGOKM to RPE GOKM to RPE
Slide 39: Ananda Labs
Ananda Labs
Slide 40: Milestone: CD34+ Stem cell rejuvenation for MDS and immune system
AnandaLabs Milestone Milestone 11 Milestone Milestone 3 Milestone 22 Milestone 3 Milestone 4 Milestone 4 CD34+ CD34+ Stem Stemcell cell rejuvenation rejuvenation for for YEAR YEAR1 YEAR YEAR 2 2 YEAR YEAR 3 3 YEAR YEAR 4 4 YEAR YEAR 5 5 Costs $ Costs €A MDS MDS and and immune system immune system Q1
Slide 41: AnandaLabs
AnandaLabs.
Slide 42: Thank You!
Thank You! 0 é 1111 010 è 111 00 DD 011 CHIIRME 191 0 1DHOUT 1210 OHI DNI A% WI AUOHOGIOROO ome* 00.0 DM: LA trio oorin PODOnO CIRCOD 001 ADODC1p1 0D11 1000000UDcIoniS, 1 CUT a 011 0111101 HI D 411 AnandaLabs:209