HTG Molecular Diagnostics, Inc. · OTC:HTGMQ
HTG Molecular Diagnostics — Technical Presentation (2020)
15 slides from HTG Molecular Diagnostics's technical presentation, 2020
Slide 1: Analysis of the microenvironment of follicular lymphoma by gene expression analysis: importance of the Th17 axis
Analysis of the microenvironment of follicular lymphoma by gene expression analysis: importance of the Th17 axis PD Dr. med. Thomas Menter DGP Congress 2020 DGP05.06
Slide 2: Lymphomas and their microenvironment
Lymphomas and their microenvironment Double-edged relationship of lymphomas and their TME • Dependence of trophic/supportive substances and structures of lymphoma cells • Manipulation of the immune system by lymphoma cells to avoid recognition by the immune system Menter et al. Pathobiology 2020
Slide 3: The SAKK 35/10 trial
The SAKK 35/10 trial • Cooperation of the SAKK and the Nordic Lymphoma Group • Evaluation of the addition of Lenalidomide to Rituximab (R2) in treatment-naive follicular lymphoma in comparison to Rituximab- therapy only • Enrollement of 154 patients • Primary endpoint: complete remission in week 23
Slide 4: Conclusions of the first study part
Conclusions of the first study part • Analysis of the TME by immunohistochemistry focusing on T-cell subsets • Insights into the heterogeneity of the tumor microenvironment of follicular lymphoma • Microenvironment simplified: already a small subset analysis of the microenvironmental T-cells yields
Slide 5: Heterogeneity of the CD4/CD8 ratio
Heterogeneity of the CD4/CD8 ratio A C E CD4 CD4 CD4 CD4 CD4 B D F CD8 CD8 CD8 CD8 CD8
Slide 6: Lenalidomide overcomes the prognostic importance of PD1+ TIL
Lenalidomide overcomes the prognostic importance of PD1+ TIL Menter et al. Br J Haematol 2020
Slide 7: Positive influence of GATA3+ T-cells in the lenalidomide group
Positive influence of GATA3+ T-cells in the lenalidomide group Menter et al. Br J Haematol 2020
Slide 8: Gene expression profiling
Gene expression profiling • Analysis of remaining 71 cases with sufficient material • Gene expression profiling using the HTG EdgeSeq Precision Immuno-Oncology Panel • Additional immunohistochemistry for Th17 cells (RORγt)
Slide 9: Cluster analysis
Cluster analysis TH17 high TH17 low
Slide 10: Progression-free survival analysis
Progression-free survival analysis Rituximab only Rituximab+Lenalidomide
Slide 11: RORγt+ T-cells in follicular lymphoma
RORγt+ T-cells in follicular lymphoma
Slide 12: Progression-free survival analysis
Progression-free survival analysis Rituximab+Lenalidomide
Slide 13: Role(s) of Th17 cells
Role(s) of Th17 cells BioMed Research International Volume 2015, Article ID 314620
Slide 14: Conclusions
Conclusions • Correlation between immunohistochemical analysis and gene expression profiling data • Similar to the role of GATA3- and PD1-positive T-cells, the prognostic role of Th17 cells depends on the treatment given • Potential mode of action: induction of Th17 cells and downregulation of regul
Slide 15: [email protected]
The Power of | Diagnostics Pathology Basel [email protected]
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