Altria Group, Inc. · NYSE:MO

Altria — Technical Presentation (September 2019)

14 slides from Altria's technical presentation, Sep 17, 2019

  1. Slide 1: Preclinical Testing of Flavors in E-vapor Products Part 4: Flavor Transfer from Liquid to Aerosol

    Preclinical Testing of Flavors in E-vapor Products Part 4: Flavor Transfer from the Liquid to the Aerosol for Inhalation Exposure Jingjie Zhang Tobacco Science Research Conference September 17, 2019 Jingjie Zhang | Regulatory Affairs | Altria Client Services l TSRC Sep 17, 2019 I FINAL 1

  2. Slide 2: Overview

    Overview  Preclinical testing of flavors in e-vapor products, Part 1: selection of representative flavor mixtures for toxicological evaluations using a structural grouping approach  Preclinical testing of flavors in e-vapor products, Part 2: preparation and stability characterization of representa

  3. Slide 3: Overview

    Overview Preclinical Application Part 3 In-vitro Pre-start Part 4 Aerosol Exposure In-vivo PG: propylene glycol VG: vegetable glycerol Jingjie Zhang | Regulatory Affairs | Altria Client Services l TSRC Sep 17, 2019 I FINAL 3

  4. Slide 4: Objective

    Objective  To generate aerosols using designated test formulations  To characterize the generated aerosols  To confirm the flavor transfer from formulation to aerosols Jingjie Zhang | Regulatory Affairs | Altria Client Services l TSRC Sep 17, 2019 I FINAL 4

  5. Slide 5: Aerosol Generation

    Aerosol Generation  Test Formulation with and without nicotine CAG: Capillary Aerosol Generator Jingjie Zhang | Regulatory Affairs | Altria Client Services l TSRC Sep 17, 2019 I FINAL 5

  6. Slide 6: Aerosol Size Distribution

    Aerosol Size Distribution  Method - Cascade impactor Test Formulation w/ Test Formulation w/o Nicotine Nicotine (n = 4) (n = 4) MMAD (µm) 0.97 ± 0.07 1.23 ± 0.06 GSD 1.77 ± 0.18 1.82 ± 0.13 Jingjie Zhang | Regulatory Affairs | Altria Client Services l TSRC Sep 17, 2019 I FINAL 6

  7. Slide 7: Aerosol Sampling for Chemical Analysis

    Aerosol Sampling for Chemical Analysis Analyte of Interest Cambridge Filter Pad Impinger Aerosol Mass 44 mm No Nicotine, PG, Glycerin 44 mm No Selected Carbonyls 44 mm DNPH derivatization solution Selected Flavors 55 mm Ethanol (-70°C) pH No Fritted; KCl solution Mass Flow CAG Vacuum Controller Mixi

  8. Slide 8: Analytical Characterization

    Analytical Characterization Test Formulation w/ Nicotine (N = 3) Test Formulation w/o Nicotine (N = 3) Analyte Liquid Aerosol Transferb Liquid Aerosol Transferb Aerosol Mass (mg) NA 98.1±2.0 NA NA 108.2±1.8 NA Ethanol (mg/g) 20.44±0.13 BLOQ NA 20.19±0.23 BLOQ NA Glycerol (mg/g) 144.3±0.3 146.2±2.1a

  9. Slide 9: Flavor Transfer

    Flavor Transfer  Test formulations contained 38 flavors.  22 out of 38 (Lab 1) were analyzed in both the liquid and the aerosol. Jingjie Zhang | Regulatory Affairs | Altria Client Services l TSRC Sep 17, 2019 I FINAL 9

  10. Slide 10: Selected Carbonyls in the Aerosol

    Selected Carbonyls in the Aerosol Carrier Blank High w/ Nicotine High w/o Nicotine (PG/VG/Nicotine/ (n = 3) (n = 3) (n = 3) Water) (n = 3) Aerosol Mass (mg) 100 mg 107.2 ± 5.4 106.7 ± 1.3 116.1 ± 1.5 Formaldehyde (µg/g) c < LOQ 8.71 ± 0.57 4.98 ± 0.15 5.88 ± 0.24 Acetaldehyde (µg/g) c 3.09 ± 0.11 8.

  11. Slide 11: Acetaldehyde as Measured by DNPH Derivatization

    1,1-Diethoxyethane in Pre-blend Ic Detected as Acetaldehyde Impinger for carbonyl sampling  1,1-diethoxyethane H [H+] detected as acetaldehyde O O 2,4-DNPH in the carbonyl analysis 1,1-Diethoxyethane Acetaldehyde - 2, 4-DNPH (Acetal; CAS: 105-57-7) due to the sampling limitation Approximation (abov

  12. Slide 12: pH of the Aerosol

    pH of the Aerosol Test Formulation w/o Nic Test Formulation w/ Nic Liquid pH (n = 3) 4.6 7.7 Aerosol pH (n = 3) 4.7 7.6 pH adjustment in the formulation? Test Formulation w/o Nic (pH adj. w/ NaOH) Liquid pH (n = 1) 7.1 Aerosol pH (n=3) 4.6 Always characterize the test atmosphere After <1 hr for conf

  13. Slide 13: Summary

    Summary  Flavor transfer from liquid formulation into the aerosol was confirmed.  Particle size for both formulations (high with and without nicotine) tested were in the desired range (MMAD<1.6 µm, GSD<2).  Nicotine, PG and glycerol matched in formulations and CAG aerosol for the test formulation

  14. Slide 14: Acknowledgements

    Acknowledgements Altria Client Services, Richmond, Virginia, USA Cameron Smith Chase Anderson Nicholas McCutcheon John Miller K. Monica Lee Kimberly Ehman l Regulatory Affairs l Altria Client Services l TSRC Sept 17, 2019 l Final 14

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